ࡱ> { qvbjbjzz 4Kn%LL8=d2H"jjjEEE!=#=#=#=#=#=#=$?AG=+EE++G=jj\==6=6=6+jj!==6+!==6=6:U9,9j@&dao19 =r=0=9 @B-5@B9@B9xEl=6G"%EEEG=G=5EEE=++++@BEEEEEEEEEL l: Rolf Joseph Craven 2429 Woodfield Circle Lexington, Kentucky 40515 Phone (859) 245-4108 Office address: University of Kentucky Department of Molecular and Biomedical Pharmacology 213 Combs Building, Markey Cancer Center Lexington, Kentucky 40536 Phone (859) 323-3832 FAX (859) 257-9608 e-mail  HYPERLINK "mailto:rolf.craven@uky.edu" rolf.craven@uky.edu Education: B.S. Biochemistry Clemson University Clemson, S.C. April, 1985 M.S. Biochemistry University of Illinois- Chicago, Ill. December, 1987 Chicago Health Sci. Center (Mentor: Dr. Stephen S. Chung) Ph.D. Genetics University of North Chapel Hill, N.C. December, 1996 Carolina-Chapel Hill (Mentor: Dr. Edison T. Liu) Professional experience: Research Analyst UNC-Chapel Hill Chapel Hill, NC 8/1990-8/1993 Department of Surgery, Lineberger Cancer Center Post-doctoral fellow UNC-Chapel Hill Chapel Hill, NC 8/1996-12/2000 Department of Biology (Dr. Thomas Petes, member Natl. Acad. Sci.) Assistant Professor UNC-Chapel Hill Chapel Hill, NC 1/2001-6/2003 Department of Surgery, Division of Surgical Oncology Assistant Professor University of Kentucky Lexington, Ky. 7/2003-present Department of Molecular and Biomedical Pharmacology Associate Professor University of Kentucky Lexington, Ky. 5/2009-present Department of Molecular and Biomedical Pharmacology Vice-Chair, IRB University of Kentucky Lexington, Ky. 6/2013-present Scientific Advisory Board Cognition Therapeutics, Inc. Pittsburgh, PA. 6/2013-present Faculty Member University of Kentucky Lexington, Ky. 6/2013-present Graduate Center for Nutritional Sciences Areas of expertise: Development of experimental therapeutics/target identification and validation Receptor-mediated and intracellular signaling in proliferation, survival, and adhesion Mechanisms of apoptosis and resistance in cancer cells Gene expression and manipulation in clinical tumor samples and tumor cell lines Awards: 2007 Wethington Award for research from the University of Kentucky 2006 Wethington Award for research from the University of Kentucky 2004 Wethington Award for research from the University of Kentucky 2001-3/2007-8 Scholar of the B.I.R.C.W.H. Program (Building Interdisciplinary Research Careers in Womens Health) from the N.I.H. Post-doctoral fellowship from the American Cancer Society. "Biological functions of the ATM-related gene TEL1 Rhone-Poulenc Travel Award for the 87th Annual Meeting of the American Association of Cancer Research. Washington, D.C. Publications: Mir, S.U.R., Schwarze, S., Jin, L., Zhang, J. Friend, W., Miriyala, S., St Clair, D. and Craven, R.J. Sigma-2 receptor associates with MAP1-LC3B and promotes autophagy. Autophagy, in press. Jin, L. and Craven R.J. The Rak/Frk tyrosine kinase associates with and internalizes the epidermal growth factor receptor. Oncogene, in press. Breuer, E.-K. Y., Murph, M.M. and Craven, R.J. Biochemical Pathways in Cancer. Biochemistry Research International. 2012: 268504 (2012). Mir, S.U.R., Jin, L. and Craven, R.J. Ngal (neutrophil gelatinase-associated lipocalin) transcription dependent on the tumor-associated sigma-2 receptor S2RPgrmc1. Journal of Biological Chemistry, 287: 14494-14501 (2012). Ahmed, I.S., Chamberlain, C. and Craven, R.J. S2RPgrmc1: the cytochrome-related sigma-2 receptor that regulates lipid and drug metabolism and hormone signaling. Expert Opinion on Medicinal Chemistry and Toxicology, 8: 361-370 (2012). Mir, S.U.R., Ahmed, I.S., Arnold, S. and Craven, R.J. Elevated Pgrmc1 (progesterone receptor membrane component 1)/sigma-2 receptor levels in lung tumors and plasma from lung cancer patients. International Journal of Cancer 131: 1-9 (2011). Mallory, J.C. and Craven, R.J. Candida albicans Dap1p promotes ergosterol synthesis via the P450 protein Erg11p/Cyp51p, regulating susceptibility to azole antifungal drugs, morphogenesis and damage resistance. Pharmacologia 3: 179-189 (2011). Ahmed, I.S., Rohe, H.J., Twist, K.E. and Craven, R.J. Pgrmc1 (progesterone receptor membrane component 1) associates with EGFR and regulates erlotinib sensitivity, Journal of Biological Chemistry, 285: 24775-24782 (2010). Ahmed, I.S, Rohe, H.A., Twist, K., Mattingly, M. and Craven, R.J. Pgrmc1 (progesterone receptor membrane component 1): a heme-1 domain protein that promotes tumorigenesis and is inhibited by a small molecule. Journal of Pharmacology and Experimental Therapeutics, 333: 564-573 (2010). Dietrich CS, Greenberg VL, DeSimone CP, Modesitt SC, van Nagell JR, Craven R., Zimmer SG. Suberoylanilide Hydroxamic Acid (SAHA) Potentiates Paclitaxel-Induced Apoptosis in Ovarian Cancer Cell Lines. Gynecologic Oncology, 116: 126-130 (2010). Yim, E.-Y., Peng, G., Dai, H., Hu, R., Li, K., Lu, Y., Mills, G.B., Meric-Bernstam, F., Hennessy, B.T., Craven, R.J. and Lin, S.-Y. Rak functions as a tumor suppressor by regulating PTEN protein stability and function. Cancer Cell, 15: 304-314 (2009). Craven, R.J., Rohe, H.A., Ahmed, S.A. and Twist, K. The PGRMC1 protein family: targetable receptor/co-activator proteins linking steroid hormone signaling to P450 activation. Pharmacology and Therapeutics 121: 14-29 (2009) Yang, M.M., Jarrett, S., Craven, R.J. and Kaetzel, D.M. YNK1, the yeast homolog of human metastasis suppressor NM23, is required for repair of UV radiation- and etoposide-induced DNA damage. Mutation Research, 660: 74-79 (2009). Craven, R.J. PGRMC1: a new biomarker for estrogen-responsive breast cancers. Breast Cancer Research, 10: 113-114 (2008). Craven, R.J., Mallory, J.C., and Hand, R.A. Regulation of iron homeostasis mediated by the heme-binding protein Dap1 (damage resistance protein 1) via the P450 protein Erg11/Cyp51. Journal of Biological Chemistry 282: 36543-36551 (2007). Ranney, M.K., Ahmed, I.A., Potts, K.R. and Craven, R.J. Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer. Biochimica and Biophysica Acta 1772: 1103-11 (2007). Kaetzel, D.M., Zhang, Q., Yang, M., McCorkle, J.R., Ma, D., and Craven, R.J. Potential roles of 3-5 exonuclease activity of NM23-H1 in DNA repair and malignant progression. J. Biomembranes and Bioenergetics 38: 163-167 (2006). Crudden, G., Smalley, R., and Craven, R.J. Hpr6 (heme-1 domain protein) regulates the susceptibility of cancer cells to chemotherapeutic drugs. Journal of Pharmacology and Experimental Therapeutics 316: 448-455 (2006). Mallory, J.C., Crudden, G., Oliva, A., Saunders, C., Stromberg, A., and Craven, R.J. A novel group of genes associated with survival in breast cancer cells treated with anti-neoplastic drugs. Molecular Pharmacology 68: 1748-1757 (2005). Mallory, J.C., Crudden, G., Johnson, B.L., Mo, C., Pierson, C.A., Bard, M., and Craven, R.J. Dap1p: a heme-binding protein that regulates the cytochrome P450 protein Erg11p/Cyp51p in Saccharomyces cerevisiae. Molecular and Cellular Biology, 25: 1669-1679 (2005). Crudden, G., Lsel, R., and Craven, R.J. Overexpression of the cytochrome P450 activator Hpr6 (heme-1 domain protein/human progesterone receptor) in tumors. Tumor Biology, 26: 142-146 (2005). Park, H.B., Golubovskaya, V.M., Xu, L., Yang, X., Lee, J.W., Scully, S., Craven, R.J., and Cance, W.G. Activated Src elevated adhesion, survival and alpha-2 integrin expression in human breast cancer cells. Biochemical Journal, 378: 559-567 (2004). Kurenova, E., Xu, L.-H., Yang, X., Baldwin, A.S., Craven, R.J., Hanks, S.K., Liu, Z.-G., and Cance, W.G. Focal adhesion kinase suppresses apoptosis by binding to the death domain of receptor interacting protein. Molecular and Cellular Biology, 24: 4361-4371 (2004). Hand, R.A., Jia, N., Bard, M.A., and Craven, R.J. Saccharomyces cerevisiae Dap1p, a novel DNA damage response protein related to the mammalian membrane-associated progesterone receptor. Eukaryotic Cell 2: 121-132 (2003). Craven, R.J.* and Cance, W.G. A decade of tyrosine kinases: from gene discovery to therapeutics. Surgical Oncology 12: 39-49 (2003). *corresponding author. Yang, X..H., Hand, R.A., Livasy, C.A., Cance, W.G., and Craven, R.J. Overexpression of the receptor tyrosine kinase tie-1 intracellular domain in breast cancer. Tumor Biology 24: 61-69 (2003). Hand, R.A. and Craven, R.J. The Hpr6.6 protein mediates cell death from oxidative damage in MCF-7 human breast cancer cells. Journal of Cellular Biochemistry, 90: 534-547 (2003). Meyer, T., Xu, L., Chang, J., Liu, E.T., Craven, R.J., and Cance, W.G. The Rak tyrosine kinase inhibits growth of human breast cancer cells. International Journal of Cancer 104: 139-146 (2003). Beviglia, L., Golubovskaya, V., Xu, L., Yang, X., Craven, R.J., and Cance, W.G. The focal adhesion kinase amino-terminal domain induces loss of adhesion and apoptosis in breast carcinoma cells. Biochemical Journal 373: 201-210 (2003). Craven, R.J., Greenwell, P.W., Dominska, M., and Petes, T.D. Control of genetic stability by the Saccharomyces cerevisiae kinases Mec1p and Tel1p. Genetics 161: 493-507 (2002). Golubovskaya, V., Beviglia, L., Xu, L., Earp, H.S., Craven, R., and Cance, W. Focal Adhesion Kinase (FAK) and Epidermal Growth Factor Receptor (EGFR) synergistically suppress death receptor-mediated apoptosis in human breast cancer cells by activating Akt and Erk 1 and 2 signaling pathways. Journal of Biological Chemistry 277: 38978-38987 (2002). Craven, R.J., and Petes, T.D. The Saccharomyces cerevisiae Suppressor of Choline Sensitivity (SCS2) gene is a multicopy suppressor of mec1 telomeric silencing defects. Genetics 158: 145-154 (2001). Xu, L.-H., Yang, X., Bradham, C.A., Brenner, D.A., Baldwin, A.S., Craven, R.J., and Cance, W.G. The focal adhesion kinase suppresses transformation-associated, anchorage-independent apoptosis in human breast cancer cells. Journal of Biological Chemistry 275: 30597-30604 (2000). Craven, R.J., and Petes, T.D. Involvement of the checkpoint protein Mec1p in silencing of gene expression at telomeres in Saccharomyces cerevisiae. Molecular and Cellular Biology, 20: 2378-2384 (2000). Craven, R.J., and Petes, T.D. Dependence of the regulation of telomere length on the type of sub-telomeric repeat in the yeast Saccharomyces cerevisiae. Genetics 152: 1531-1541 (1999). Xu, L.H., Yang, X., Craven, R.J., and Cance, W.G. The COOH-terminal domain of the focal adhesion kinase induces loss of adhesion and cell death in human tumor cells. Cell Growth & Differentiation 9: 999-1005 (1998). Owens, L.V., Xu, L., Dent, G.A., Yang, X., Sturge, G.C., Craven, R.J., and Cance, W.G. The focal adhesion kinase as a marker of invasive potential in differentiated human thyroid cancer. Annals of Surgical Oncology 3: 100-105 (1996). Xu, L., Owens, L.V., Sturge, G.C., Yang, X., Liu, E.T., Craven, R.J., and Cance, W.G. Attenuation of the Expression of the Focal Adhesion Kinase Induces Apoptosis in Tumor Cells. Cell Growth & Differentiation 7: 413-418 (1996). Craven, R.J., Cance, W.G., and Liu, E.T. The nuclear tyrosine kinase Rak associates with the product of the Retinoblastoma tumor susceptibility gene, pRb. Cancer Research, 55: 3969-3972 (1995). Craven, R.J., Xu, L., Weiner, T.M., Fridell, Y.-W., Dent, G.A., Srivastava, S., Varnum, B., Liu, E.T., and Cance, W.G. Receptor tyrosine kinases expressed in metastatic colon cancer. International Journal of Cancer 60: 791-797 (1995). Owens L.V., Xu, L., Craven, R.J., Dent, G.A., Weiner, T.M., Kornberg, L., Liu, E.T., and Cance, W.G.Overexpression of the focal adhesion kinase (p125FAK) in invasive human tumors. Cancer Research 55: 2752-2755 (1995). Cance, W.G., Craven, R.J., Bergman, M., Xu, L., Alitalo, K., and Liu, E.T. Rak, a novel nuclear tyrosine kinase expressed in epithelial cells. Cell Growth and Differentiation 5: 1347-1355 (1994). Craven, R.J. and Cance, W.G. Protein tyrosine kinases in the early detection of colorectal cancer. In Early detection of cancer: molecular markers Eds. Srivastava, S., Lippman, S.M., Hong, WK, and Mulshine, J.L. Armonk: Futura Publishing Co., Inc., 1994. Levedakou, E.N., He, M., Baptist, E.W., Craven, R.J., Cance, W.G., Welcsh, P.L., Simmons, A., Naylor, S.L., Leach, R.J., Lewis, T.B., Bowcock, A., and Liu, E.T. Two novel human serine/threonine kinases with homologies to the cell cycle regulating Xenopus MO15, and NimA kinases: cloning and characterization of their expression pattern. Oncogene 9: 1977-1988 (1994). Weiner, T.M., Liu, E.T., Craven, R.J., and Cance, W.G. Expression of growth factor receptors, the focal adhesion kinase, and other tyrosine kinases in human soft tissue tumors. Annals of Surgical Oncology 1: 18-27 (1994). Weiner, T.M., Liu, E.T., Craven, R.J., and Cance, W.G. Expression of focal adhesion kinase gene and invasive cancer. Lancet 342: 1024-1025 (1993). Cance, W.G., Craven, R.J., Weiner, T.M., and Liu, E.T. Novel protein kinases expressed in human breast cancer. International Journal of Cancer 54: 571-577 (1993). Cance, W.G., Craven, R.J., and Liu, E.T. Expression polymerase chain reaction: a sensitive method for analysis of gene expression in human tumors. Surgical Oncology 1: 309-314 (1992). Meetings (only selected abstracts since beginning at U.Ky. are shown): Mir, S.U.R., Jin, L. and Craven, R.J. Elevated sigma-2 receptor/Pgrmc1 (progesterone receptor membrane component 1) levels as a potential cancer biomarker in tumors and plasma. American Association for Cancer Research Annual Meeting. Chicago, Illinois, April, 2012. Mir, S.U.R., Jin, L. and Craven, R.J. Ngal (neutrophil gelatinase-associated lipocalin) transcription dependent on the tumor-associated cytochrome Pgrmc1 American Association for Cancer Research meeting on Metabolism and Cancer. Baltimore, Maryland, October 16-19, 2011. Craven RJ, Ahmed IS, Rohe HJ, Twist K and Kim JA. Pgrmc1: a tumor-associated cytochrome and therapeutic target for cancer. Conference of the Molecular Therapeutics of Cancer Research Association. Princeton University, Princeton, New Jersey, July 18, 2010. Ahmed IS, Rohe HJ, Twist KR, Kim JA and Craven RJ. Pgrmc1 associates with EGFR in the endoplasmic reticulum lumen to promote lung cancer growth and metastasis. Kentucky Lung Cancer Research Program Annual Meeting. Lexington, Kentucky, March 25, 2010. Ahmed IS, Rohe HJ, Twist KR, Kim JA and Craven RJ. Pgrmc1 promotes NSCLC migration, survival and tumor growth and associates with EGFR Kentucky Lung Cancer Research Program Annual Meeting. Louisville, Kentucky, November 14, 2009. Ahmed IS, Twist KE, Rohe HJ and Craven RJ. A novel pathway regulates EGFR (epidermal growth factor receptor) stability in cancer cells. Cold Spring Harbor Laboratory Meeting on Phosphorylation and Disease. Cold Spring Harbor, New York. May 8, 2009. Craven, R.J., Ahmed, I., Mallory, J.C. and Rohe, H. The PGRMC1/Dap1 protein family: P450-binding proteins that regulate P450 stability. 2008 Southwest Cytochrome P450 Meeting. Camp Allen, Texas. May 12, 2008. R.C. was an invited speaker. Ahmed, IA, Condley, CA and Craven, RJ. PGRMC1/Dap1: a novel family of P450 activators related to cytochrome b5. Ohio Valley Society of Toxicology Annual Meeting. Indianapolis, IN. November 2, 2007. I.A. was an invited speaker. Mallory, JM, Raymer, S, and Craven, RJ. Candida albicans Dap1 activates Erg11 and regulates antifungal drug resistance. 47th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC). Chicago, IL. September 17-20, 2007. Mallory, JC, Hand, RA and Craven, RJ. Dap1p, a Saccharomyces cerevisiae protein that regulates resistance to azole drugs and the DNA damaging agent MMS. 13th Annual Midwest Microbial Pathogenesis Conference. Cincinnati, OH. October 21, 2006. Craven RJ, Ranney M, and Ahmed I. The anti-apoptotic protein clusterin is induced by chemotherapy, stabilized by the proteasome, and degraded by intracellular proteases. American Association for Cancer Research meeting on molecular diagnostics in cancer therapeutic development. Chicago, IL. September 12-15, 2006. Ahmed I, Ranney M, and Craven RJ. Clusterin is induced by cancer chemotherapy and suppresses histone deacetylase inhibitor-induced apoptosis in breast cancer. American Association for Cancer Research meeting on molecular diagnostics in cancer therapeutic development. Chicago, IL. September 12-15, 2006. Craven RJ and Crudden G. The Hpr6.6 protein is overexpressed in human tumors and functions as a survival signal. Annual meeting of the American Association for Cancer Research. Orlando, FL. April 2, 2004. Craven RJ, Crudden G, and Johnson, BL. Hpr6 (heme-1 domain protein): a novel chemotherapy resistance protein overexpressed in human cancer cells. Sixth Annual Midwest DNA repair symposium. Lexington, Ky. June 2004. Invited seminars: University of Cincinnati, hosted by the Department of Environmental Sciences. The seminar was entitled, A novel pathway regulates EGFR (epidermal growth factor receptor) function in cancer cells. April 7, 2010. University of Miami-Ohio, hosted by the Department of Biology and Zoology. The seminar was entitled, A novel pathway regulates EGFR (epidermal growth factor receptor) function in cancer cells. September 17, 2009. Wright State University, hosted by the Department of Pharmacology and Toxicology. The seminar was entitled, Pgrmc1: a cytochrome-related membrane signaling activator induced in cancer cells. March 5, 2009. Vanderbilt University, hosted by the Department of Biochemistry. The seminar was entitled, Dap1/PGRMC1: conserved P450 activators that link cholesterol synthesis and cancer therapeutics. October 24, 2008. Duke University, The Thursday Series, co-hosted by the Department of Cell Biology, the Department of Molecular Genetics and Microbiology and the Cell and Molecular Biology Program. The title of the seminar was Conserved P450 activators that link cholesterol synthesis and cancer therapeutics. June 5, 2008. Indiana University-Purdue University at Indianapolis, Department of Biology. The seminar was entitled, Dap1 and Hpr6: novel activators of cytochrome P450 proteins. November 17, 2004. Patent: U.S. patent number 7342100, Hpr6 mutants and uses thereof, patents the use of Hpr6/Pgrmc1 mutants to sensitize cancer cells to chemotherapy. Patent application pending, A secreted tumor-associated cytochrome as a blood-based biomarker for cancer protects the discovery of Pgrmc1 as a plasma-based biomarker for cancer. Teaching: Course director: PHA622, Systems Pharmacology Additional lectures: Course: Course #: Subject of lectures: Systems Pharmacology PHA622 Respiratory and Gastrointestinal drugs Dental Pharmacology OBI836 Cancer chemotherapy, antifungal drugs and anti-tuberculosis drugs Medical Pharmacology PHA824 Antifungal drugs, gastrointestinal drugs, antihistamines, anti-tuberculosis drugs, cancer chemotherapy, gene therapy, treatment of anemia Cancer Biology IBS616 Telomeres, telomerase, and aging Genetics IBS605 Genetic analysis in yeast Cell Biology IBS603 Molecular basis of cancer Guest lecturer: Transylvania University, Senior Microbiology Course, April 2007 Past/current undergraduate, graduate and medical students: Ikhlas Said Ali Ahmed, graduate student, Pharmacology Cora Chamberlain, Research project as undergraduate, 2011 Rachel Edwina Chitti, Masters degree in Pharmacology Clay Adams Condley, medical student, did research as M2 Woodrow Friend, Graduate student, Pharmacology Ji Ae Kim, Research project as undergraduate student, enrolled in ɫy School of Pharmacy 2011 Julia Mallory, Post-doctoral fellow Shakeel Mir, Post-doctoral fellow Kelly Potts, medical student, did research as M1 Melissa Ranney, medical student, did research as M2 Hannah Rohe, medical student, did research as M1 Katie Twist, medical student, did research as M2 University service: Chair, Recruitment Committee for Director of Clinical Pharmacology, College of Medicine, 2013 Member, Faculty Recruitment Committee for Department of Molecular and Biomedical Pharmacology, three tenure-track faculty positions, 2013 Member, Institutional Review Board (IRB), 2011-present Vice-Chair, Institutional Review Board, 2013-present Member, College of Medicine Curriculum sub-committees: Foundations of Basic Science Committee, 2011-present Member, College of Medicine Curriculum sub-committees: Lymph Node/Marrow/Blood Committee, 2011-present Member, College of Medicine Integrated Biomedical Sciences Curriculum Revision Task Force, 2011-present Member, Markey Research Conference Advisory Committee, 2008-2011 Member, Admissions Committee, Integrated Biomedical Sciences Curriculum, 2007-2011 Member, American Cancer Society Institutional Research Grant Oversight Committee, 2008-2011 Judge, U. Ky. School of Medicine Post-doctoral Research Day Member, Graduate Education Steering Committee, Department of Molecular and Biomedical Pharmacology, 2007-present Director, IBS student orientation for the Pharmacology Department, 2007-2011 Reviewer, Project grant, Marty Driesler Cancer Project Reviewer, Project grant, Markey Cancer Center Reviewer, Pilot grant project, University of Kentucky Research Foundation Doctoral thesis committee member for the following current graduate students: Lin Ao, Pharmaceutical Sciences Tripti Bhattarai, Toxicology Leann Fiore, Pharmacology Anwesha Goswami, Pharmaceutical Sciences SangHee Lee, Nutrition Sciences Xiaobo Li, Biochemistry Cassandra Reiling, Toxicology Jason Tucker, Pharmacology Doctoral thesis committee member for the following former graduate students: Ikhlas Ahmed, Pharmacology Meng Meng Cheng, Pharmacology Rachel Smalley Chitti, Pharmacology Kedra Cyrus, Pharmacy Aubrey Franz, Microbiology and Molecular Genetics Brock Howerton, Chemistry Xiuling Li, College of Pharmacy Enerlyn Lozada, Toxicology Michelle Olson, Pharmacology Dinesh Puppula, Pharmacology Timothy Scott, Toxicology Jonathan Sims, Pharmacology Yolanda Williams, Pharmacy Katerina Zaytseva, Pharmacology Advisor for the following Integrated Biomedical Sciences rotation students: Chirie Sumanasekera Megan Sampley Janine Owens Lesley Gilmer Angie Martin Rachel Smalley Brandon Adkins Ikhlas Said Ali Ahmed Lorenzo Federico Matthew Thacker Memberships: American Association for Cancer Research American Society for Microbiology American Society for Pharmacology and Experimental Therapeutics American Society for Biochemistry and Molecular Biology Ohio Valley Society of Toxicology Extramural study section: Department of Defense- CDMRP- Scientist Reviewer- Clinical and Experimental Therapeutics A- 2010-present American Institute for Cancer Research- Ad hoc reviewer 2012 Dutch Cancer Society- Ad hoc reviewer- 2012 Reviewer for the following journals: Biochemistry Editorial Board, Biochemistry Research International Breast Cancer Research British Journal of Cancer Cellular and Molecular Life Sciences Chemistry and Biology (Cell press) Comparative Hematology Eukaryotic Cell European Journal of Biochemistry International Journal of Cancer Journal of Pharmacology and Experimental Therapeutics Journal of Biological Chemistry Molecular and Cellular Biology Molecular and Cellular Endocrinology Molecular Pharmacology PLOS One Tissue Antigens Research support Current: Stable isotope-derived metabolomics to elucidate the mechanism of a tumor-associated cytochrome in lung cancer growth and metabolism Role: Project P.I. Agency: KLCR Type: Research award Period: 12/1/2010-11/30/2012 The goals of the project are to determine the role of the cytochrome Pgrmc1 in regulating glycolysis and hypoxia-associated metabolism in lung cancer. Novel small molecule inhibitors targeting cancer growth via EGFR Rolf: Project P.I. Agency: KSEF-09-RDE-013 research grant Period: 8/1/2010-7/30/2012 The goals of the project are to determine the extent to which Ero1 and Pgrmc1 regulate EGFR folding and to develop and test new inhibitors for Pgrmc1. S2RPgrmc1: sigma-2 receptor as a therapeutic target in lung cancer Role: Project P.I. Agency: Bonnie J. Addario Lung Cancer Research Foundation Period: 1/1/2012-12/31/2013 The goals of the project are to determine the extent to which secreted S2RPgrmc1 acts as a prognostic indicator for resistance to EGFR inhibitors. A stem cell biomarker and therapeutic target in Appalachian lung cancer Role: co-PI Agency: University of Kentucky Clinical Center for Translational Research Period: 3/1/2012-2/28/2013 The goals of the project are to isolate stem cells from clinical lung tumors at Marshall University, analyze them for S2R levels and test their sensitivity to S2R inhibitors. Pending: Pre-clinical and clinical chemotherapy with taxanes and sigma-2 receptor inhibitors Role: Project P.I. Agency: KLCR Type: Research award Period: 5/1/2012-4/30/2014 The goals of the project are to determine the activity of sigma-2 receptor inhibitors combined with standard chemotherapy against lung cancer in cell line and animal models. Completed: Signaling from receptor tyrosine kinases to PTEN in lung cancer Role: Project P.I. Agency: KLCR Type: Research award Period: 11/1/2009-10/31/2011 The goals of the project are to determine the extent to which the intracellular Rak tyrosine kinase is inhibited by receptor tyrosine kinases, de-stabilizing the tumor suppressor PTEN. A novel approach for targeting the epidermal growth factor receptor (EGFR) in lung cancer Role: Project P.I. Agency: KLCR Type: Research grant Period: 1/1/2009-12/31/2010 The goals of the project are to perform pre-clinical tests with a novel Pgrmc1 inhibitor that de-stabilizes the receptor tyrosine kinase EGFR in lung cancer cells and to test the correlation between EGFR and Pgrmc1 in expression in clinical lung cancer samples. A novel activator of Cyp19/aromatase induced in human breast cancer cells Role: project P.I. Agency: ACS Type: 85-001-19-IRG Period: 1/1/2008-12/31/2009 The primary aim of the project is to test the extent to which PGRMC1 (progesterone receptor membrane component 1) binds and activates the P450 protein Cyp19/aromatase. Gender differences in the metabolism of addictive drugs via P450 protein activation Role: Scholar Agency: NIH/NIGMS Type: BIRCWH P20 RR 15592 Period: 9/1/2007-8/31/2008 The major goal of the project is to determine the extent to which PGRMC1 is induced differentially by certain addictive drugs, altering their metabolism. Clusterin splice variants in chemotherapy resistance P.I.: Craven Agency: ACS Type: 85-001-19-IRG Period: 1/1/2006-12/31-2007 The primary aim of the project is to analyze the synthesis and function of a nuclear isoform of clusterin in breast cancer cells treated with the chemotherapeutic agent doxorubicin. Hpr6 (heme-1 domain protein): a novel regulator of progesterone and estrogen action P.I.: Craven Agency: NIH/NIGMS Type: COBRE P20 RR 15592 Period: 7/1/2004-4/30/2007 The major goal of the project is to determine the extent to which Hpr6 (an activator of P450 proteins) regulates the synthesis and signaling function of steroid hormones. Yvh1p, a regulator of Mec1p telomere signaling P.I.: Craven Agency: NIH/NIA Type: 1 R03 AG022700-01 Period: 7/1/2003-6/31/2004 The major goals of the project are to analyze the function of the Yvh1p phosphatase as a negative regulator of the Atm-related kinase Mec1p. Hpr6, a cytochrome b5-related stress protein P.I.: Orringer Agency: NIH/NICHD Type: 1 K12 HD001441 Period: 4/1/2001-4/30/2003 This was a junior faculty training grant in which the role of Hpr6 in breast cancer progression and in oxidative stress was analyzed. The role of the Atm-related TEL1 gene in telomere maintenance P.I.: Craven/Petes Agency: ACS Type: PF-4435 (post-doctoral) Period: 4/1/1997-3/30/2000 The major goals of the project were to analyze the genetic pathway regulated by the Saccharomyces cerevisiae TEL1 gene in regulating chromosome structure and telomere length maintenance. The biological role of the Rak tyrosine kinase in breast cancer P.I.: Craven/Liu Agency: DOD Type: DAMD17-96-6068 (pre-doc) Period: 6/1/1995-12/31/1996 The major goals of the project were to analyze the binding partners of the Src-related Rak tyrosine kinase in human breast cancer cells.     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